Figure 9. Rhus coriria downregulated the level of p53 in MDA-MB-231 cells.

(A) Concentration-dependent reduction in the protein level of p53 in RCE-treated cells. MDA-MB-231 cells were treated with and without increasing concentrations of RCE for 48 h and DNA p53 protein level was analyzed by Western blot. (B) Time-course measurement of p53 level in treated cells. Cells were treated with 400 μg/mL RCE and p53 level was examined at different time-point (6, 12, 24 and 48 h). (C) Effect of autophagy inhibitors on p53 protein level. Cells were pretreated with the indicated concentrations of CQ or 3-MA for 1 h before adding RCE (200 and 400 μg/mL) for 48 h. Cells were then harvested and p53 protein level was determined by western blot as described above. The western blots shown are representative of two independent experiments.