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. 2015 Aug 12;5:12999. doi: 10.1038/srep12999

Figure 1. EZH2 is overexpressed in human osteosarcoma cell lines.

Figure 1

(A) EZH2 mRNA expression was determined in hFOB1.19 osteoblasts, MTF osteoblasts and osteosarcoma cell lines (SaOS2, U2OS, MG63 and HOS) by qRT-PCR analysis. EZH2 expression was significantly increased in MTF cells compared with hFOB1.19 osteoblasts, whereas TSSC3 expression was notably decreased. Values shown are the mean ± SD from three wells from a representative of three independent experiments. ***P < 0.001; **P < 0.01; bars, SD. (B) Western blot analysis showing EZH2 and TSSC3 expression in hFOB1.19, MTF and osteosarcoma cells (upper). The results were consistent with that of qRT-PCR. Blots are representative of three experiments and were reprobed for β-actin to verify equal loading (lower). Band intensities were measured by densitometry and normalised to β-actin expression. (C) Osteosarcoma cell lines were immunostained with goat anti-EZH2 (red) and rabbit anti-TSSC3 antibody (red) and Hoechst33258 (blue) for observation by laser confocal microscopy. Scale bars: 50 μm.