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. 2015 Aug 12;5:12999. doi: 10.1038/srep12999

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Copyright © 2015, Macmillan Publishers Limited

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(A) Lenti-shEZH2 and control vector transfected cells treated with cisplatin were immunostained with the TUNEL antibody (green) and Hoechst33258 (blue), scale bars 50 μm. (B) Lenti-shEZH2 transfected cells treated with cisplatin showed higher apoptosis rates than control group, which is representative of three independent experiments. Bar graph of apoptosis (right) rate is shown. (C) Western blot analysis of cleaved caspase 9, cleaved caspase 3, and cleaved PARP proteins in osteosarcoma cells (left). Blots are representative of three experiments and were reprobed for β-actin to verify equal loading (right). Band intensities were measured by densitometry and normalised to β-actin expression. (D) CCK-8 analysis showed that after treatment with cisplatin, EZH2-silenced SaOS2 and U2OS cells showed a decrease in proliferation.