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. 2015 Aug 12;5:12999. doi: 10.1038/srep12999

Figure 7. EZH2 repressed TSSC3 expression via H3K27me3 modification and inhibition of TSSC3 partially rescues the impaired biological function of EZH2 silencing.

Figure 7

(A) Western blot analysis of HK27me3 after lenti-shEZH2 transfection using total histone 3 as a reference (left). Blots are representative of three experiments and were reprobed for histone 3 to verify equal loading (right). Band intensities were measured by densitometry and normalised to histone 3 expression. (B) ChIP analysis shows the H3K27me3 levels within the TSSC3 promoter, which is inhibited by EZH2 silencing. IgG was utilised as a negative control. (C) EZH2 silencing lead to a G1 phase cell cycle arrest, which was decreased by TSSC3 knockdown. (D) Soft agar assays. (E) Apoptosis assays. (F) TUNEL assays. (G) Apoptosis assays are applied to indicated cells treated with cisplatin. (H) Transwell matrigel invasion assay. Scale bars: 50 μm.