Figure 3.

Differential gene expression between HepG2-pu6 and HepG2-siPK cells. A: The number of statistically significant differentially expressed transcripts identified from the F-test. Color plots show the expression levels of significantly differentially expressed transcripts (P < 0.05 between groups and false discovery rate 0.05) between HepG2-pu6 and HepG2-siPK cells. The upregulated genes are indicated in red, and the downregulated genes are indicated in green. Genes that did not show a statistically significant difference are indicated in blue; B: The graph shows gene expression in relation to cell migration and invasion. Fold differences were calculated based on the log2 ratio of siPK/pu6; C: A comparison of the RT-qPCR and RNA sequence expression analysis; D: The expression levels of genes that were determined to have statistically significant differences in expression were analyzed by immunoblot analysis in stable HepG2 and Huh-7 cells.