FIG. 1.

Isoflurane reduced fetal growth and development. (A) Anesthesia with 1.4% isoflurane for 2 h daily for 3 days reduced fetal growth and development. Ctrl means the fetal mouse from a pregnant mouse without treatment. Isoflurane means fetal mouse from a pregnant mouse treated with 1.4% isoflurane. (B) Isoflurane reduced fetal growth compared with fetal weights×number of fetuses between the control group and isoflurane group. Data shown are mean±SD (n=20). *P<0.05. (C) Detection of gene expression in the mouse brain by qRT-PCR. Data shown are mean±SD (n=6). *P<0.05, **P<0.01, ***P<0.001. SD, standard deviation; qRT-PCR, reverse transcription-real time PCR. Color images available online at www.liebertpub.com/scd