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Effect of the KYD pattern-serum on calcified nodules in hFOB 1.19 cells (magnification, x100). (A and B) Cultivation of cells on day 14; the hFOB 1.19 cells were treated with the (A) KYD pattern-serum or (B) control serum for 14 days. (C and D) Alizarin red S staining for calcified nodules: (C) KYD pattern-serum-treated cells and (D) control serum-treated cells. KYD, Kidney-Yang deficiency.
