Restriction enzyme digestion of recombinant expressed plasmid Echinococcus granulosus glutathione S-transferase (GST)/pET-28a by EcoRI and NotI. Lane 1, recombinant plasmid; lane 2–3, GST/pET-28a digested by EcoRI and NotI; lane 4, polymerase chain reaction-amplified product; lane 5, 200-bp DNA marker; lane 6, λDNA/HindIII marker.