Table 1. The demographic and clinical characteristics of the enrolled patients and healthy subjects.

Clinical parameter	Sequencing group (n = 4 per group)			Validation group (n = 30 per group)
	HBV-ACLF	CHB	Healthy	HBV-ACLF	CHB	Healthy
Age (years)	33.8 ± 10.2	41.8 ± 8.3	30.8 ± 7.6	45.6 ± 10.1	38.8 ± 11.1	28.4 ± 4.3
Sex (M/F)	4/0	4/0	1/3	24/6	21/9	16/14
ALB (g/L)	33.1 ± 3.6	47.3 ± 2.9	48.7 ± 3.8	30.5 ± 3.8	48.9 ± 2.0	47.7 ± 2.3
ALT (U/L)	489.5 ± 125.7	28.5 ± 5.9	12.5 ± 3.7	386.8 ± 294.0	30.6 ± 19.2	13.6 ± 8.2
AST (U/L)	412.3 ± 20.8	23.3 ± 2.1	16.8 ± 2.6	311.7 ± 229.5	29.9 ± 24.4	16.8 ± 3.1
TB (μmol/L)	568.0 ± 92.6	12.3 ± 4.3	9.8 ± 1.7	363.7 ± 110.9	16.4 ± 6.5	15.5 ± 14.3
Cr (μmol/L)	85.3 ± 23.5	67.3 ± 18.8	62.8 ± 24.2	70.1 ± 20.1	66.9 ± 12.3	63.2 ± 17.2
Sodium (μmol/L)	133.5 ± 2.4	141.5 ± 1.3	139.8 ± 1.0	136.4 ± 4.0	141.2 ± 1.8	139.8 ± 1.1
INR	2.22 ± 0.27	1.10 ± 0.15	NA	2.07 ± 0.37	0.97 ± 0.05	NA
HBV DNA log10(IU/ml)	6.8 ± 7.0	7.1 ± 7.2	NA	7.8 ± 8.5	8.0 ± 8.2	NA
MELD	27.0 ± 2.4	NA	NA	26.1 ± 2.5	NA	NA