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. 2015 Aug 12;5:13038. doi: 10.1038/srep13038

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Copyright © 2015, Macmillan Publishers Limited

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(a) The extent of syncytium formation is shown for representative BHK-21F monolayers expressing pCAGGS (pCG) vector, wtHN, or HN bearing the A89Q, P93A, or L94A substitution. Cells were fixed with 4% paraformaldehyde and stained with Giemsa. Syncytia are indicated by black arrows. (b) The fusion index of wt HN and HN mutants was calculated as the mean number of nuclei per syncytium. All values are expressed relative to the HN wt value of 100%. The mean and standard error of 20 fusion events for each HN are shown above each column (***P < 0.001).