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H2O2-induced apoptosis is enhanced in Prx1 knockdown cells. Following transfection for 48 h, cultured cells were treated with 5 mM H2O2 for 0, 15, 30 and 45 min. Apoptotic cell death was measured by flow cytometry. Data are represented as the mean of triplicate independent experiments ± standard error of the mean. **P<0.01 vs. mock. Prx1, peroxiredoxin 1; shRNA, short hairpin RNA.
