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. 2015 Jun 22;10(3):1655–1661. doi: 10.3892/ol.2015.3406

Figure 1.

Figure 1.

Rheb phosphorylation is induced by Ras overexpression. (A) HEK293 cells were transfected with 0.2 µg HA-PRAK, 0.2 µg Myc-Rheb or 0.6 µg HRas alone or in combination. Phosphorylation of Rheb was determined using anti-phospho-Rheb (S130) antibodies. (B) HEK293 cells were transfected with HA-PRAK, Myc-Rheb and increasing concentrations of HRas (0, 0.1, 0.2, 0.4 and 0.6 µg). Phosphorylated Rheb levels were then determined. p-Rheb, phosphorylated Ras homolog enriched in brain; PRAK, p38-regulated/activated protein kinase; HA, hemagglutinin.