Figure 4.

Immunohistochemical staining of head and neck squamous cell carcinoma spheroids 5 days after dissemination. (A) FaDu cells were spun onto slides, fixated and stained with Ki-67 in the monolayer model. (B) Staining of a spheroid (diameter, 300 µm) of FaDu cells with Ki-67 antibody for the 3D model. Vascular endothelial growth factor staining of the CAL27 cells in (C) monolayer and (D) spheroid cell cultures. Epithelial growth factor staining in the CAL27 cells in the (E) 2D and (F) spheroid cell models. Survivin expression of the SCC25 cells in the (G) 2D and (H) spheroid cell models. All images were captured at x100 magnification.