Figure 2.

YM155 induces cell apoptosis and proliferation arrest in HCC cell lines. (A and B) The apoptosis ratios of HepG2 and Huh7 cells were significantly elevated by YM155 treatment. (C) In MTT cell viability assays, YM155 notably suppressed the cell proliferation of cancerous hepatocytes. (D and E) YM155 significantly inhibited survivin and p-Rb protein expression, and promoted procaspase-3 expression as determined by western blotting and densitometry (relative to β-actin). (n=3, *P<0.05). HCC, hepatocellular carcinoma; p-Rb, phosphorylated retinoblastoma tumor suppressor; PI, propidium iodide; AV, Annexin V.