Table 8.
Comparison and validation of the proteomic data with earlier reported proteomic work
| Work | Brief description | Proteins identified | Proteins found in this work (iTRAQ) | Key findings | Comparison with mRNA expression |
|---|---|---|---|---|---|
| Schaffer et al. (2002) | Solventogenesis | 130 | All 130 proteins have been identified under butanol/butyrate stress | Proteins involved in the solventogenic pathway | Northern analysis (selected few) |
| Sullivan and Bennett (2006) | Acidogenesis and solventogenesis | 23 | 22 proteins were observed in this study | Proteins expressed during the onset of solventogenesis | With microarray data from Tomas and Alsaker (2004) |
| Mao et al. (2010) | Cytoplasmic proteins (DSM 1731 and Rh8 tolerant mutant) | 564 | 86 out of the 102 differentially expressed proteins were identified | Proteins that play a role in solvent toxicity tolerance | qRT-PCR of selected differentially expressed protein |
| Janssen et al. (2010) | Continuous culture of WT in acidogenic and solventogenic phases | 178 + 205 | 178 acidogenic proteins and 205 solventogenic proteins | Proteins that are expressed under different metabolic phases | DNA microarrays |
| Mao et al. (2011) | Membrane protein DSM 1731 and RH8 tolerant phenotype | 341 | 23 out of the 33 differentially expressed membrane proteins were identified | Membrane proteins that were differentially expressed in the butanol hyper-tolerant mutant | None |
| Sivagnanam et al. (2011) | CAC proteome under glucose and xylose utilization | 717 (glucose) 826 (xylose) | 22 of the 23 differentially expressed proteins were identified | Proteins that correspond to differential utilization of carbon sources | None |
| Bai and Ji (2012) | Phophoproteome of C. acetobutylicum | 61 phospho-proteins | 57/61 phosphor proteins were also identified in our work | Proteins with post translational modifications (PTMs) and their role in stress response | None |
| Sivagnanam et al. (2012) | Protein interaction network using STRING and CYTOSCAPE | 217 proteins were used to construct a PPI network with 1947 interactions | N/A | Construction of PPI network to identify regulatory interactions | None |
| Jang et al. (2014) | Acidogenesis and solventogenesis. WT, M5, and M5 + pIMP | 56 | All were also identified by us | Proteins differentially expressed during acidogenic and solventogenic phases | None |