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. 2015 Jul 24;6(1):138. doi: 10.1186/s13287-015-0126-x

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© Qin et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization and flow cytometry analysis of ADSCs. P3 GFP-ADSCs adopted a more uniform fibroblast-like, spindle-shaped population a, and GFP was observed under fluorescence microscopy b. P6 GFP-ADSCs adopted increased aging or dedifferentiated shape with irregularity e. The intensity of GFP gradually reduced under fluorescence microscopy f. Flow cytometry revealed that CD90 and Sca-1 surface antigens of P3 c, d and P6 g, h GFP-ADSCs were gradually reduced with statistically significant differences (*P <0.05) i, j. Scale bar = 100 μm. GFP green fluorescent protein, P3 passage 3, P6 passage 6