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. 2015 Jul 24;6(1):138. doi: 10.1186/s13287-015-0126-x

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© Qin et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of periostin on the apoptosis of ADSCs under hypoxia. Apoptosis of GFP-ADSCs transfected with or without periostin under hypoxia a or normoxia b was analyzed using flow cytometry analysis. The apoptosis rates c indicated that overexpression of periostin effectively enhanced the viability of ADSC. Western blotting analysis revealed that the levels of anti-apoptotic Bcl-2 increased and pro-apoptotic Bax decreased d, and RT-PCR e showed that the ratio of Bcl-2/Bax was significantly higher in P-ADSCs than in ADSCs in normoxic and hypoxic treatments. *P <0.05. ADSC adipose-derived stem cell, GFP green fluorescent protein, P-ADSC periostin-transfected ADSC