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. 2015 Jun 26;36(3):833–838. doi: 10.3892/ijmm.2015.2262

Figure 1.

Figure 1

Apoptosis inhibitor of macrophages (AIM) can interact with insulin-like growth factor binding protein-4 (IGFBP-4). (A) AIM can interact with IGFBP-4 in vitro. The purified His-HA-AIM was pre-incubated with His-IGFBP-4 for 2 h, and was subjected to co-IP using the anti-HA tag polyclonal antibody. The immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis using anti-His tag antibody. (B and C) AIM can interact with IGFBP-4 in the 293 cells. The 293 cells were co-transfected with either HA-AIM and FLAG-IGFBP-4 or HA-IGFBP-4 and FLAG-AIM. The co-IP experiments were conducted using anti-FLAG antibody. Western blot analysis was performed using the HA monoclonal antibody. The rightmost lane in each panel represents the total un-precipitated sample control. IP, immunoprecipitation; WB, western blot.