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. 2015 Jun 26;36(3):833–838. doi: 10.3892/ijmm.2015.2262

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Kinetic analysis of the interaction between apoptosis inhibitor of macrophages (AIM) and insulin-like growth factor binding protein-4 (IGFBP-4) by biolayer interferometry. The Super Streptavidin biosensor tips of the ForteBio Octet RED 96 were coated with biotinylated IGFBP-4. The biosensor tips were dipped in increasing concentrations of AIM (indicated on the right side of each binding curve) to measure binding of AIM to IGFBP-4 (left side of the graph) and subsequently moved to wells containing buffer to measure dissociation rates (right side of the graph). A representative experiment of three replicates is shown.