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. 2015 Jul 10;36(3):661–668. doi: 10.3892/ijmm.2015.2280

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Autophagy regulates the catabolic effects induced by tumor necrosis factor-α (TNF-α) in nucleus pulposus (NP) cells at the protein level. (A) Western blot analysis showed cell protein COX2, condition medium protein and matrix metalloproteinase 3 (MMP3) expression in NP cells treated with autophagy inhibitor and activator. Densitometry showed protein expression of medium (B) MMP3 protein and (C) COX2 induced by TNF-α was significantly promoted by 3-methyladenine (3-MA) and markedly repressed by rapamycin (Rapa) in NP cells. Data represent mean + standard error of three independent experiments. ^*P<0.05. CM, condition medium; Ctr, control.