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. 2015 Jul 10;36(3):661–668. doi: 10.3892/ijmm.2015.2280

Figure 6.

Figure 6

Stable silencing of IKKβ increases the autophagy-related protein expression in human nucleus pulposus (NP) cells. (A) Western blot analysis showed that the Beclin-1, LC3-II and IKKβ protein expression in NP cells was transduced with LV-shCtr or LV-shIKKβ in the tumor necrosis factor-α (TNF-α) condition. (B) Densitometric analyses of NP cells transduced with LV-shCtr or LV-shIKKβ; IKKβ expression levels were suppressed by LV-shIKKβ in the TNF-α condition. Compared to cells transduced with LV-shCtr, NP cells transduced with LV-shIKKβ demonstrated a higher expression of (C) LC3 protein turnover and an increase of (D) Beclin-1 protein in TNF-α condition. Data represent mean + standard error of three independent experiments. *P<0.05. Ctr, control.