Figure 2.

AMFR siRNA downregulates the mRNA and protein expression levels of AMFR in THP-1 cells. (A) mRNA expression of AMFR at different time-points following transfection with AMFR siRNA, detected using reverse transcription-quantitative polymerase chain reaction. β-actin was used as an internal reference. ^*P<0.05, vs. blank control; ^#P<0.05, vs. 72 h. (B) Protein expression of AMFR 72 h after transfection, detected using immunoblotting. β-actin was used as an internal reference. The data are expressed as the mean ± standard deviation of three independent experiments. ^*P<0.05, vs. blank control; ^#P<0.05, vs. scrambled siRNA. AMFR, autocrine motility factor receptor; siRNA, small interfering RNA.