Effects of AMFR siRNA or pcDNA3.1-AMFR-C on the protein expression levels of ROCK2, Bcl-2 and cyclin D1 in THP-1 cells. (A) Protein expression levels of ROCK2, Bcl-2 and cyclin D1 following AMFR siRNA transfection for 72 h, detected using immunoblotting. *P<0.05, vs. blank control; #P<0.05, vs. scrambled siRNA. (B) Protein expression levels of ROCK2, Bcl-2 and cyclin D1 following transfection with pcDNA3.1-AMFR-C for 72 h, detected using immunobloting. *P<0.05, vs. pcDNA3.1 group. The data are expressed as the mean ± standard deviation of three independent experiments. AMFR, autocrine motility factor receptor; ROCK, rho-associated, coiled-coil containing protein kinase 2; Bcl, B-cell lymphoma.