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. 2015 Aug 13;12:126. doi: 10.1186/s12985-015-0354-9

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© Wang et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Construction of recombinant pDEV^C-KCE-H5(UL55). a A kanamycin resistance gene (sm) with a 40 bp homologous sequence (c) was inserted into the Sac I restriction site (*) in the HA expression cassette, which was divided into two parts (HA Cas’ and HA Cas”). b The HA expression cassette with the selective marker (sm) was inserted into the noncoding area between LORF11 and UL55 through the first recombination to generate a recombinant BAC clone (pDEV^C-KCE-H5(UL55)^KAN) with both chloramphenicol and kanamycin resistance. c The second recombination was performed to delete the kanamycin resistance gene and generate the final recombinant pDEV^C-KCE-H5(UL55)-vectored HA clone. Rectangles with the same type of shading indicate identical sequences. Scales in bp or kbp are provided