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. 2015 Aug 12;15:582. doi: 10.1186/s12885-015-1574-5

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© El-Mashed et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Western blot analysis of human recombinant microtubule-associated proteins 1A/1B light chain 3A and 3B probed with either a the Abgent anti-LC3 antibody (AP1802a), or b MBL anti-LC3A/B antibody. LC3 bands were quantified using the Odyssey Infrared Imaging System (Li-Cor), and data presented as integrated intensities (n = 3). c Analysis of LC3B distribution in untreated OE21 and OE33 esophageal cancer cell lines. Arrows indicate diffuse cytoplasmic LC3B expression (magnification 400×) (n = 6). d Analysis of LC3B distribution in untreated KYSE450 and OE19 esophageal cell lines. Arrows indicate the presence of LC3B crescent or ring-like (upper panels) and globular (lower panels) structures in KYSE450 and OE19 cells (magnification 1000×) (n = 6)