Table 1.
Recommended Printing Parameters
| Step | Parameter | Description | Recommended Value |
|---|---|---|---|
| Sample Loading | Pin depth | Distance pin is submerged into source solution | 5 mm |
| Speed | Speed pin is lowered into the source solution | 4 mm/s | |
| Dwell time | Time pin held in source solution before wiggles or dips | 1 second | |
| Number of wiggles | Times pin moved laterally while submerged | 0 | |
| Number of dips | Times pin moved vertically while submerged | 2 | |
| Dip height | Distance pin is further submerged | 2 mm | |
| Inter-dip delay | Time pin kept submerged between dips | 1 second | |
| Lid Handling | Specifies when lid is replaced | Immediately | |
| Pre-Spotting | Number of pre-spots | Times a freshly loaded pin is spotted onto a slide before it is used to print slides that will be used for experiments | 18 |
| Delay before pre-spotting | Time pin is momentarily suspended above slide before descending to contact the slide | 0.5 second | |
| Target height | Distance tool is lowered to bring pin into contact with slide | 1.5 mm | |
| Speed | Speed that tool is lowered to bring pin into contact with slide | 4 mm/s | |
| Surface Height | Thickness of slide | 1 mm | |
| Dwell time | Time tool is held in place at its target height | 0.250 second | |
| Multiple Strikes | Number of times the pin is tapped onto the slide to form each spot | 1 | |
| Pitch | Distance pin moves laterally or vertically between pre-spots | 0.45 mm | |
| Printing | Number of print slides | High-print quality slides that will be used for experiments | 12 |
| Delay before spotting | Time pin is momentarily suspended above slide before contact | 0 second | |
| Target height | Distance tool is lowered as pin contacts slide | 1 mm | |
| Speed | Speed that tool is lowered as pin contacts slide | 4 mm/s | |
| Dwell time | Time tool is held in place at its target height | 0 second | |
| Multiple Strikes | Number of times the pin is tapped onto the slide to form each spot | 1 | |
| Number of spots/sample | Number of spots per samples | 2-4 spots | |
| Pitch | Distance pin moves laterally or vertically between spots | 0.2 mm | |
| Washes | Dip Height | Distance pins submerged into recirculating baths | 5 mm |
| Bath Dwell Time | Time pins are held in bath | 3 seconds | |
| MWS Washes | Times wash in Main Wash Station (MWS) is repeated | 2 | |
| Fill Time | Time that water is pumped into MWS | 3 seconds | |
| Drain Time | Time that vacuum is turned on to drain MWS | 8 seconds | |
| Number of Wash Cycles | Number of times the entire wash cycle is repeated | 2 |