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Recommended buffers and starting dilutions of samples for incubation on glycan microarray
| Sample Type | Incubation Buffer | Concentration | Conditions |
|---|---|---|---|
| Lectin | 1% BSA in PBST | 1-50 μg/mL | RT for 2 hrs |
| Monoclonal Antibody | 3% BSA in PBST | 1-50 μg/mL | 37 °C while gently shaken for 2-4 h |
| Serum Antibodies | 3% BSA in PBST | 1:50 to 1:200 | 37 °C while gently shaken for 4 h |
