Table 3.

Recommended Secondary Reagents and Conditions

Sample Type	Secondary Label	Buffer	Concentration	Conditions
Biotinylated Lectin	Cy3-Streptavidin	1% BSA in PBS buffer	2 μg/mL	RT for 2 hrs
Monoclonal Antibody	Monoclonal antibody specific for species and isotype of 1° Ab	3% BSA in PBS	2 μg/mL	37 °C while gently shaken for 2 h
Human Serum Antibodies	Monoclonal anti-human IgG, IgM, and/or IgA	3% HSA + 1% BSA in PBS	2 μg/mL	37 °C while gently shaken for 2 h