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. 2015 Apr 15;20(4):6670–6682. doi: 10.3390/molecules20046670

Figure 2.

Figure 2

Purification of mincle CRD by affinity chromatography. Renatured mincle was applied to a 10-mL column of trehalose-Sepharose. Following washing with 10 mL of buffer containing Ca2+, the column was eluted with EDTA-containing buffer in 1-mL fractions. Aliquots (15 μL) of the final fraction collected during the wash (W) along with the elution fractions were analyzed by SDS-polyacrylamide gel electrophoresis. Protein was visualized by Coomassie blue staining. The expected molecular weight of the CRD with the biotinylation tag is 17.8 kDa.