Figure 6.

Sequence alignment and conservation of key residues in the CRD of mincle across species. A selection of the 62 sequences of mincle orthologs spanning a range of species is shown. Some of the key conserved framework residues common to C-type CRDs are highlighted in yellow, while the residues involved in binding are indicated as green at the primary binding site for the first residue of glucose in trehalose, magenta at the secondary binding site for the second glucose residue in trehalose and blue at the hydrophobic groove that is proposed to form a binding site for acyl groups attached to the 6-OH groups of trehalose.