Skip to main content
. Author manuscript; available in PMC: 2015 Aug 12.
Published in final edited form as: Nat Struct Mol Biol. 2012 Aug 12;19(9):930–937. doi: 10.1038/nsmb.2356

Figure 3.

Figure 3

H2BpY37 suppresses the transcription of histone genes located in the Hist1 cluster. (a) qRT-PCR of RNA prepared from synchronized MEFs indicating a rapid decrease in histone mRNA levels after 6.5 h after thymidine release. The y axis label “histone/actin” indicates the amount of histone transcript compared to that of actin. Error bars indicate the standard error. (b) Synchronized MEFs were harvested, stained with propidium iodide and then subjected to flow cytometry, which indicated that cells were in the late S/G2 phase at 6–8 h after thymidine release. (c–m) qRT-PCR of RNA prepared from synchronized MEFs indicating an increase in histone mRNA levels at 8.5 h in samples treated with WEE1 inhibitor compared to untreated samples. The histograms include data from two independent biological experiments, each done in triplicates. (n) The nuclei were prepared from synchronized MEFs treated with the WEE1 inhibitor MK-1775 (0.625 µM, 14 h) or left untreated. Run-on assay was performed, revealing that WEE1 regulates histone transcription. Data in all the bar graphs are the mean ± s.d. Data are representative of two independent biological experiments.