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. 2015 Jul 21;6(1):132. doi: 10.1186/s13287-015-0131-0

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© Mao et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Quantification of isobaric H3K27-R40me5 in mESCs and differentiated cells. a SIMC quantification of K27me3-K36me2 (green) and K27me2-K36me3 (red) isoforms of H3K27-R40me5 in histones from mESCs (D0) and at differentiation day 2 (D2) and day 4 (D4) induced by LIF withdrawal. b Relative abundance of each isoform of H3K27-R40me5 in mESCs and differentiated cells normalized to the H3D123-R128 peptide. The levels of H3K27-R40me5 and H3D123-R128 peptides were determined by SIC, and the amount of K27me2-K36me3 and K27me3-K36me2 isoforms was calculated by multiplying the total level of the H3K27-R40me5 by SIC and the constituent percentage of each isoform by SIMC. The MS experiment was repeated three times and the error bars represent the SD. ** P <0.01