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. 2015 Aug 13;8:113. doi: 10.1186/s13068-015-0296-x

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© Chung et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Chromosomal integration of the E1 gene into the C. bescii genome. a A diagram of native E1 protein: SP, signal peptide; a family 5 endoglucanase; and CBM2, a family 2 carbohydrate-binding module/domain. The black bar beneath the diagram represents the portion of the pDCW175 construct derived from Acel_0614. b A depiction of the chromosomal location and integration event of the E1 expression cassette/secretion cassette. c Agarose gel showing PCR products amplified using primers DC477 and DC478 annealing to regions outside the site of integration in the newly constructed strain JWCB052 ΔpyrFA ldh::ISCbe4 Δcbe1::P_S-layer acel0614 E1 containing the E1 expression cassette, 4.079 kb (lane 2) and the parent strain, JWCB018 ΔpyrFA ldh::ISCbe4 Δcbe1, 2.440 kb (lane 3); DNA MW standards (lane 1); no template PCR control (lane 4).