Table 1.
Strains and plasmids used in this work
| Strains/plasmids | Strain and genotype/phenotype | References |
|---|---|---|
| C. bescii | ||
| JWCB001 | Wild type (ura +/5-FOAS) | DSMZa |
| JWCB018 | ΔpyrFA ldh::ISCbe4 Δcbe1 (ura −/5-FOAR) | [29] |
| JWCB052 | ΔpyrFA ldh::ISCbe4 Δcbe1::PS-layer acel0614 (E1) b (ura -/5-FOAR) | This study |
| Escherichia coli | ||
| JW314 | DH5α containing pDCW144 (ApramycinR) | [27] |
| JW336 | DH5α containing pDCW174 (ApramycinR) | This study |
| JW337 | DH5α containing pDCW175 (ApramycinR) | This study |
| Plasmids | ||
| pDCW144 | Integrational vector for Caldicellulosiruptor bescii (ApramycinR) | [27] |
| pDCW174 | Integrational vector containing signal peptide of CelA for Caldicellulosiruptor bescii (ApramycinR) | This study |
| pDCW175 | Integrational vector containing the PS-layer acel0614 (E1) b expression cassette (ApramycinR) | This study |
a German Collection of Microorganisms and Cell Cultures.
b acel0614 (E1) [glycoside hydrolase (family 5) with cellulose-binding (family 2) derived from Acidothermus cellulolyticus 11B].