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. Author manuscript; available in PMC: 2015 Aug 12.
Published in final edited form as: Cell Biochem Funct. 2011 Aug 9;29(7):589–596. doi: 10.1002/cbf.1792

Figure 2. SHKBP1 competed with c-Cbl to bind to CIN85 through its two PXXXPR motifs.

Figure 2

a HEK293T cells co-transfected with expression plasmids encoding Myc-c-Cbl, Flag-SHKBP1 or Flag-CIN85 were lysed 48 hours after transfection and cell lysates were immuno-precipitated with anti-Myc antibody and control mIgG. Immuno-precipitated proteins were processed for western-blot with anti-Flag antibody and the membrane was stripped and re-blotted with anti-Myc antibody.

b HEK293T cells co-transfected with plasmids encoding Myc-c-Cbl, Myc-SHKBP1 and Flag-CIN85 were lysed 48 hours after transfection and cell lysates were immuno-precipitated with anti-Flag antibody and control mIgG. Immuno-precipitated proteins were processed for western-blot with anti-Myc antibody and the membrane was stripped and re-blotted with anti-Flag antibody.

c HEK293T cells co-transfected with plasmids encoding Myc-c-Cbl, Myc-R623A/684A and Flag-CIN85 were lysed 48 hours after transfection and cell lysates were immuno-precipitated with anti-Flag antibody and control mIgG. Immuno-precipitated proteins were processed for western-blot with anti-Myc antibody and the membrane was stripped and re-blotted with anti-Flag antibody.