. Author manuscript; available in PMC: 2015 Aug 12.

Published in final edited form as: Cell Biochem Funct. 2011 Aug 9;29(7):589–596. doi: 10.1002/cbf.1792

Table 1. Yeast two-hybrid experiment examining the interaction of SHKBP1 or its mutants with CIN85.

MAV203 yeast strain was co-transformed with SHKBP1/pDBLeu or its mutants plasmids and CIN85/pPC86 plasmids. SHKBP1 or its mutants co-transformed with empty pPC86 and CIN85 co-transformed with empty pDBLeu were used as control. And pPC86 and pDBLeu vectors were used as negative control. Transformed yeasts were selected in SC-Leu-Trp plates, and a LacZ assay was performed with colonies grown on the SC-Leu-Trp plates and control yeast strains: a strong positive control A, a weak positive control C and a negative control supplied by manufacturer. (‘+++’ indicates a strong positive signal, ‘+’ indicates a weak positive signal and ‘−’ indicates a negative signal).

Plasmids	SHKBP1	SHKBP1- R623A	SHKBP1- R684A	SHKBP1- R623A/684A	pDBLeu
Plasmids	CIN85	CIN85	CIN85	CIN85	pPC86
LacZ activity	+++	+++	+	−	−
Plasmids	SHKBP1	SHKBP1- R623A	SHKBP1- R684A	SHKBP1- R623A/684A	pDBLeu
Plasmids	pPC86	pPC86	pPC86	pPC86	CIN85
LacZ activity	−	−	−	−	−