Table 3.
Strains and plasmids used in this study
| Strains/plasmids | Function and relevant characteristics | Sources |
|---|---|---|
| Strains | ||
| DH5α | General clone host strain | Invitrogen |
| C. crenatum MT | Mutation strain with auxotrophic for biotin, and producing l-Arg | Lab stock |
| CCM01 | C. crenatum MT with proB gene deletion, which E-argB gene replaced by C. crenatum argB M4 gene (E19R, H26E, D311R, and D312R) | This work |
| CCM02 | Chromosomal deletion of the Ncgl1221 gene of CCM01 | This work |
| CCM03 | Chromosomal deletion of the dtsR1 gene of CCM02 | This work |
| Plasmids | ||
| pK18mobsacB | Mobilizable vector, allows for selection of double crossover in C. crenatum, KmR, sacB | Lab stock |
| pK18-ΔNCgl1221 | A derivative of pK18mobsacB, harboring ΔNCgl1221 fragment | This work |
| pK18-ΔdtsR1 | A derivative of pK18mobsacB, harboring ΔdtsR1 fragment | This work |