Table 4.

Sequences of oligonucleotide primers

Primers	Sequence (5′–3′)	Purposes
NCgl1221-up-F	CGCAAGCTTCAAGAAAGCCCTCGTTCCAACACTG	Amplifying the left arm of NCgl1221
NCgl1221-up-R	ATCAGCGTCCTAAGGAATCAAAAACGCCAAGACCAGG
NCgl1221-down-F	GGCGTTTTTGATTCCCTTAGGACGCTGATTACAGACG	Amplifying the right arm of NCgl1221
NCgl1221-down-R	GCATCTAGAGGAAGGGAGTTGAAGGTGACG
dtsR1-up-F	CGCAAGCTTCAGCAAGTCAGCATTAGTGGAGC	Amplifying the left arm of dtsR1
dtsR1-up-R	GCCGATTTACAGTGTGAAATCGTAGCGG TAGG
dtsR1-down-F	CCGCTACGATTTCACACTGTAAATCGGCGAATCC	Amplifying the right arm of dtsR1
dtsR1-down-R	GCATCTAGAGGAAGGGAGTTGAAGGTGACG
odhA-F	TCATTGAGGCATACCGCTCC	RP of odhA
odhA-R	TGAAGGTACGGTCCAGATCC
lpdA-F	TGGGACTTAACCGTGGGCT	RP of lpdA
lpdA-R	CACACCGTTAATCATGCGGAC
sucB-F	TCGTGAAGCGTCCAGTTGTC	RP of sucB
sucB-R	GGTCCTTGATGGTGGTCAGG
pknG-F	GGCGGCATGGGTTGGATTT	RP of pknG
pknG-R	GTGCCTTGGTCTTGAACGGA
ppp-F	CGGTATTGATCGTAGCCCTG	RP of ppp
ppp-R	CGCGACGTAAAAAGTGCTG
dtsR1-F	CCTCCCATCCAACAATCGCT	RP of dtsR1
dtsR1-R	TAAGGAACGGTCGCGGAATC
zwf-F	ACTGAGATTGCCGTGGTGTT	RP of zwf
zwf-R	AGCGGATGAGCACACCTTC
argB-F	GTCGCGATTTAGTTGGTTTGAT	RP of argB
argB-R	GAGGCATCGACATTAATGATGTCT
16s rRNA-F	AAGAAGCACCGGCTAACTAC	Reference Gene
16s rRNA-R	CCGGGATTTCACAGACGAC

Restriction sites were highlighted in bold; linker sequences for crossover PCR were shown in underline.

RP RT-qPCR.