Table 3.

Collection media type and room temperature incubation times evaluated in the study

Media tested	Incubation time 0	Incubation time 1	Incubation time 2
		(72 h at 25 °C)	(168 h at 25 °C)
No media	3 Aliquots	–	–
RNAlater®	3 Aliquots	3 Aliquots	3 Aliquots
RNAlater® + Kana	3 Aliquots	3 Aliquots	3 Aliquots
RNAlater® + Ciprob	3 Aliquots	3 Aliquots	3 Aliquots

Study participants (n = 10) sampled aliquots (n = 30) from a single stool which they stored under different conditions. Subsamples were collected either without collection media or three different RNAlater-based media and frozen immediately after collection (time 0) or stored by two time periods (72 and 168 h) at 25 °C before freezing on dry ice and stored at −80 °C

^aRNAlater® supplemented with 300 μg/ml Kanamycin

^bRNAlater® supplemented with 300 μg/ml Ciprofloxacin