(A1-A2): transfection efficiency and cell viability at different MC (100 μl/ml, 200 μl/ml, 300 μl/ml and 400 μl/ml) when AI was 0.5W/cm2. (B1-B2): transfection efficiency and cell viability at different MC (100 μl/ml, 200 μl/ml, 300 μl/ml and 400 μl/ml) when AI was 1.0 W/cm2. (C1-C2): transfection efficiency and cell viability at different MC(100 μl/ml, 200μl/ml, 300μl/ml and 400μl/ml) when AI was 1.5W/cm2. (D1-D2) transfection efficiency and cell viability at different MC (100 μl/ml, 200 μl/ml, 300 μl/ml and 400 μl/ml) when AI was 2.0 W/cm2. Indication: Symbols indicate significant differences (P < 0.05) from the 100μg/ml. (*), from 200μg/ml (†) and from 300μg/ml (‡)respectively.