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. 2015 Jul 21;6(1):131. doi: 10.1186/s13287-015-0129-7

Table 1.

List of antibodies used for the phenotypical characterization of Hipp-NSCs and hNIS-Hipp-NSCs

Primary antibody Specificity Dilution Source
Mouse anti-nestin Neural progenitor cells 1:5000 WB Millipore
1:200 IF
Rabbit anti-SOX2 Neural progenitor cells 1:2000 WB Abcam
1:100 IF
Mouse anti-βIII-tubulin Immature and mature neurons 1:5000 WB Promega
1:1000 IF
Mouse anti-NeuN Mature neurons 1:2000 WB Millipore
1:1000 IF
Rabbit anti-GFAP Astrocytes 1:5000 WB Dako
1:1000 IF
Mouse anti-oligodendrocyte specific protein Oligodendrocytes 1:200,000 IF Millipore
Mouse anti-hNIS Human NIS 1:100 IF Abcam

Hipp-NSC hippocampus-derived neural stem cell, hNIS-Hipp-NSC hippocampus-derived neural stem cell expressing the human sodium iodide symporter, WB Western blot, IF immunofluorescence, anti-GFAP anti-glial fibrillary acidic protein