Table 1.
Primary antibody | Specificity | Dilution | Source |
---|---|---|---|
Mouse anti-nestin | Neural progenitor cells | 1:5000 WB | Millipore |
1:200 IF | |||
Rabbit anti-SOX2 | Neural progenitor cells | 1:2000 WB | Abcam |
1:100 IF | |||
Mouse anti-βIII-tubulin | Immature and mature neurons | 1:5000 WB | Promega |
1:1000 IF | |||
Mouse anti-NeuN | Mature neurons | 1:2000 WB | Millipore |
1:1000 IF | |||
Rabbit anti-GFAP | Astrocytes | 1:5000 WB | Dako |
1:1000 IF | |||
Mouse anti-oligodendrocyte specific protein | Oligodendrocytes | 1:200,000 IF | Millipore |
Mouse anti-hNIS | Human NIS | 1:100 IF | Abcam |
Hipp-NSC hippocampus-derived neural stem cell, hNIS-Hipp-NSC hippocampus-derived neural stem cell expressing the human sodium iodide symporter, WB Western blot, IF immunofluorescence, anti-GFAP anti-glial fibrillary acidic protein