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. 2015 Aug 13;8:420. doi: 10.1186/s13071-015-1037-5

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© Yan et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Impairment of parasitic motility impeded NO-induced egress of T. gondii from HFFs. NO-induced egress after pre-treatment with cytochalasin D (a) or DMEM (b) was observed by microscopy. Intact parasitophorous vacuole (black arrow), lytic parasitophorous vacuole (white arrow). Bar: 20 μm. c Tachyzoite- infected HFFs were pre-treated with cytochalasin D before NO-induced egress assay. Egressed parasites were suspended with 500 μL of DMEM, and 20 μL suspensions were analysed by flow cytometry. The data in the figure show the means ± SEMs of five replicates and represent three independent experiments, **P < 0.01