Table 3.

ANOVA results of sugar and protein from seahorse for the yield of chondroitin sulfate

Source	Sum of squares		df	Mean square		F Value		P-value Prob > F
	Saccharide	Protein		Saccharide	Protein	Saccharide	Protein	Saccharide	Protein
Model	0.23	0.49	14	0.016	0.035	2.65	7.94	0.039	0.0002
A-Time	0.018	8.841E-004	1	0.018	8.841E-004	2.99	0.20	0.1.59	0.6623
B-Salt	0.049	0.015	1	0.049	0.015	8.14	3.45	0.0128	0.0843
C-Tem	0.030	0.35	1	0.030	0.35	4.95	78.40	0.0430	<0.0001
D-Ratio	0.012	2.028E-003	1	0.012	2.028E-003	1.93	0.46	0.1859	0.5102
AB	4.624E-003	6.250E-006	1	4.624E-003	6.250E-006	0.76	1.407E-003	0.3970	0.9706
AC	5.522E-004	1.323E-004	1	5.522E-004	1.323E-004	0.091	0.030	0.7671	0.8655
AD	4.202E-004	4.622E-004	1	4.202E-004	4.622E-004	0.069	0.10	0.7961	0.7517
BC	3.062E-004	2.500E-005	1	3.062E-004	2.500E-005	0.051	5.629E-003	0.8253	0.9413
BD	0.011	1.156E-003	1	0.011	1.156E-003	1.75	0.26	0.2069	0.6179
CD	1.369E-003	2.103E-004	1	1.369E-003	2.103E-004	0.23	0.047	0.6418	0.8309
A2	0.041	5.880E-003	1	0.041	5.880E-003	6.69	1.32	0.0215	0.2691
B2	0.011	0.042	1	0.011	0.042	1.75	9.55	0.2070	0.0080
C2	4.320E-003	0.097	1	4.320E-003	0.097	0.71	21.91	0.4125	0.0004
D2	0.074	1.029E-004	1	0.074	1.029E-004	12.19	0.023	0.0036	0.8812
Residual	0.085	0.062	14	6.057E-003	4.441E-003
Lack of Fit	0.031	0.058	10	3.099E-003	5.758E-003	0.23	5.01	0.9728	0.0671
Pure Error	0.054	4.597E-003	4	0.013	1.149E-003
Cor Total	0.31	0.56	28

The value of Prob > F (a) represents difference between the model and each factor; when it is >0.05, there is no significant effect; when it is <0.05, there is a difference or effect, and <0.01 means a significant effect.