Table 1.
The Molecular Masses, and Measured and Theoretical Collisional Cross Sections (CCS) for USP5 and Its Ubiquitin Complexes
| Protein complex | Molecular Mass/Da | Mean CCS/nm2 | |
|---|---|---|---|
| Experimentala | Theoretical | ||
| USP5 | 93,792 | 50.2 (±1.9) | 53.5 |
| USP5•mono-Ub | 102,357 | 54.7 (±0.9) | 57.2,b 56.9c |
| USP5•mono-Ub•mono-Ub | 110,922 | 59.0 (±0.7) | — |
| USP5•ΔG75/G76-mono-Ub | 102,243 | 55.2 (±0.7) | — |
| USP5•tetra-Ub | 127,980 | 66.1 (±1.4) | — |
a
Mean CCS are derived from the IM drift-trace peak maxima for each charge state detected (see Supporting Information Fig. S7-9 for drift trace plots). Errors are ± standard deviation.
b
ZnF-UBP-bound.
c
catalytic site-bound.
- indicates not calculated.