Figure 3.

Mass binning can bisect isotopic distributions in a deuteration-dependent manner. (A) Edges of a given mass bin, here indicated by the vertical line, can exert a variable effect on a changing deuteration pattern. A peptide affected by bin edges can be useable in MS space but will have perturbed deuteration values in MS² space. (B) Edging probability is a function of percent D₂O applied, and can be minimized by overlapping bins. The black box marks the conditions used in this study (35% D₂O, 1 Th overlap) and an estimate of the maximum data loss resulting from edging effects.