Table 1.
Deuteration Values from Parent Peptide and Sequence Fragments as Surrogates
| %D complexa | %D freea | ΔD | P value | |
|---|---|---|---|---|
| LVNCADDVIKML (2+)b | 17.5 (0.6) | 25.2 (1.1) | −7.7 | 0 |
| y11 (1+) | 15.1 (3.8) | 28.4 (2.6) | −13.3 | 0.00 |
| y10 (1+) | 19.3 (0.6) | 26.1 (1.2) | −6.8 | 0.00 |
| y9 (1+) | 18.5 (1.8) | 25.7 (1.0) | −7.2 | 0.00 |
| y8 (1+) | 17.1 (2.0) | 25.8 (2.6) | −8.7 | 0.00 |
| y7 (1+) | 23.3 (1.6) | 26.3 (2.0) | −3.0 | 0.04 |
| b9 (1+)ML | 19.8 (2.5) | 26.3 (1.7) | −6.5 | 0.01 |
| b8(1+)IKML | 21.6 (3.7) | 26.6 (2.2) | −5.0 | 0.07 |
| b7 (1+)VIKML | 22.5 (1.2) | 26.6 (2.4) | −4.1 | 0.02 |
a
Value (std. dev.) in percentage of maximum peptide deuteration.
b
Peptide or sequence fragment (charge states).