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. 2015 Aug 12;10(8):e0135499. doi: 10.1371/journal.pone.0135499

Fig 2. APP and Aβ peptide expression.

Fig 2

Fluorescent immunolabeling of APP in 6 months old (A, B) and 48 months old (C, D) retinal sections. DAPI (blue) stains nucleus. GS labeling in adult retina (E). Aβ peptide expression in young (F, G) and adult (H, I) animals. The insert shows a magnification of the NFL-GCL area. Thin arrows indicate labeling in inner retina. Large arrows specify labeling in the GCL area. White lines on the left hand side of the image indicate the extent of the IPL. The number of pixels occupied by the antibody in the NFL-GCL is shown (J). Slot blot assay showing the level of Aβ protein detection with Aβ6E10 for young and adult degus (K). APP protein level was assayed using western blot (L). APP expression as a function of age was quantified in (M). Abbreviations: glutamine synthetase (GS), nerve fiber layer- ganglion cell layer (NFL-GCL), pixels (px). Scale bar = 20 μm.