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. 2015 Aug 12;10(8):e0135499. doi: 10.1371/journal.pone.0135499

Fig 3. Expression of Aβ peptides.

Fig 3

Fluorescent and enzyme-linked immunolabeling show Aβ4G8 labeling of Aβ peptides in 6 months old (A-C) and 48 months-old (D-F) retinal sections. DAPI (blue) stains nucleus. White lines on the left hand side of the image indicate the extent of the IPL. Aβ peptides were also labeled using fluorescence immunohistochemistry in the adult central (I, J) and peripheral (K, L) GCL. Arrows indicate the location of Aβ deposits. Negative control immunolabeling without the antibody is (G, H). The number of pixels occupied by the Aβ4G8 antibody in the central and peripheral NFL-GCL is shown (M). Slot blot assay for detection of Aβ peptides as a function of age (N) was quantified in (O). Abbreviations: nerve fiber layer- ganglion cell layer (NFL-GCL), pixels (px). Scale bar = 20 μm.