Fig 5. Tau and PHF-tau protein expression.

Fluorescent immunolabeling shows tau expression in 6 months old (A, B) and 48 months old (C, D) retinal sections. DAPI (blue) stains nucleus. PHF-tau proteins labeled with AT8 in young (E, F) and adult (G, H) retinal sections. The insert shows a magnification of the NFL-GCL. Large arrows indicate positive labeling in the GCL area and thin arrows highlight the labeling pattern along the vertical axis, which is reminiscent of Müller cells. Thin white lines on the left hand side of the image indicate the extent of the IPL. Whole mount labeling using Tau5A6 (I, J) and AT8 in the adult central GCL (K, L). Arrows indicate the location of tau and phosphorylated tau respectively. The number of pixels occupied by the Tau5A6 and AT8 antibodies in the central NFL-GCL is shown (M). Western blot of phosphorylated tau and PHF levels in young and adult retinae using Ser235 and AT8 antibodies (N). Quantification of the bands shows an age-related increase in expression of phosphorylated tau and PHF detected with Ser235 and AT8 (O). Anti-actin was used as loading control. Scale bar = 20 μm