Fig 4. Total protein O-linked β-N-acetylglucosamine (O-GlcNAc) levels, and the regulatory enzymes glutamine-fructose-6-phosphate transaminase 2 (GFAT2), O-GlcNAc transferase (OGT), and O-GlcNAcase (OGA).

Molecular weight markers (in kDa) for the O-GlcNAc western and protein staining are shown next to the respective images. OGT and OGA each had two bands, which corresponded to the molecular weights for known isoforms. Of note, the OGA western required different exposure times to identify both isoforms. Sham is the Cont-Sham group. n = 3 Sham, n = 5 Cont-TAC and n = 5 MycKO-TAC. * p<0.05 between the indicated group versus Sham, # p<0.05 Cont-TAC versus MycKO-TAC.